The Columbia Genome Center is equipped with the IN Cell Analyzer 2000 (GE Healthcare), a high-throughput imaging system for performing high-content analysis.
The instrument is equipped with a large CCD camera (resolution 2048 x 2048 pixels) that is capable of whole-well imaging in 96- or 384-well microplates. The IN Cell 2000 has several imaging modes, including image restoration and deconvolution. This enables users to get confocal-like quality in a high-throughput experimental setting. The instrument is also configured with environmental and temperature control and liquid handling modules to conduct real-time live cell imaging.
High-content image analysis software (IN Cell Investigator) is also integrated into this platform. It includes several image analysis modules (Object Intensity, Nuclear Trafficking, Plasma Membrane Trafficking, Granularity, Cell Cycle Trafficking, Morphology, Dual Object, Neurite Outgrowth, Micro Nuclei, Multi-Target Analysis) which can be implemented either online during image acquisition or after a run is complete. The software uses the graphing and filtering tools of Spotfire Decision Site and allows for up to four independent users to perform the analysis simultaneously.
The IN Cell Analyzer 2000 is integrated with Cytomat microplate managing stations via a Mitsubishi robotic manipulator. This enables users to perform high-throughput screens of up to 180 microplates per set.